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antibodies against p akt  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antibodies against p akt
    Antibodies Against P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 17187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/abs+against+phospho+akt/Phospho-Akt+(Ser473)+XP+Rabbit+mAb/pmc13022637-139-20-24
    Average 99 stars, based on 17187 article reviews
    antibodies against p akt - by Bioz Stars, 2026-09
    99/100 stars

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    other:

    Article Title: Insulin-sensitizing activities of tanshinones, diterpene compounds of the root of Salvia miltiorrhiza Bunge.
    Article Snippet: In this study, the effects of the extract and four tanshinone compounds from the dried root of Salvia miltiorrhiza Bunge (Labiatae) on the tyrosine phosphorylation of the insulin receptor (IR) b-subunit and the downstream signaling were examined in Chinese-hamster ovary cells expressing human insulin receptors (CHO/IR cells) as well as in 3T3-L1 adipocytes.. In addition the translocation of the glucose transporter 4 was investigated in 3T3-L1 adipocytes.. Total extract of Danshen (1–10 mg/ml) and the four tanshinones (10 mM) did not show any activity, but the total extract and the tanshinone I, IIA and 15, 16-dihydrotanshinone I except cryptotanshinone enhanced the activity of insulin (1 nM) on the tyrosine phosphorylation of the IR as well as the activation of the downstream kinases Akt, ERK1/2, and GSK3b.

    Article Title: Role of two adaptor molecules SLP-76 and LAT in the PI3K signaling pathway in activated T cells.
    Article Snippet: Abs against phospho-Akt (pAkt, Ser473 and Thr308), phospho-Gsk3b (pGSK3b, Ser9), and phospho-FKHR (pFKHR, Ser256) were from Cell Signaling Technology (Beverly, MA).

    Staining:

    Article Title: Loss of voltage-gated hydrogen channel 1 expression reveals heterogeneous metabolic adaptation to intracellular acidification by T cells
    Article Snippet: To test viability after activation, T cells were labeled with recombinant Annexin V–AF488 and Propidium Iodide using a Dead Cell Apoptosis Kit with Alexa Fluor 488 Annexin V (Thermo Fisher Scientific) as well as FACS Abs for T cell markers as described. .. To assess intracellular signaling, fixed and permeabilized T cells were stained with primary Abs against phospho-AKT (Cell Signaling, catalog 193H12), phospho-S6 (Polyclonal, Cell Signaling), phospho-Zap-70 (Cell Signaling, catalog 65e4), and secondary donkey anti–rabbit-AF488 (Polyclonal, Life Technologies). .. To measure ROS production, purified T cells were incubated with 5 μM DHE or 2 μM CM-H2DCFDA (Thermo Fisher Scientific) before being activated by streptavidin crosslinked 1 μg/mL anti–CD3-Biotin and 5 μg/mL anti–CD28-Biotin for the indicated time points.



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    Image Search Results


    Phosphodiesterase type 5 (PDE5) inhibitor (PDE5i) administration improves insulin sensitivity in high-fat diet (HFD)-induced obese mice. (A, B) Blood glucose levels measured by insulin tolerance tests performed after vehicle or PDE5i administration for 4 and 6 weeks ( n =6). (C) Western blot data and quantification of phospho AKT, total AKT, phosphor-4E-binding protein 1 (4EBP1), total 4EBP1, phospho S6, total S6, and β-actin in gonadal white adipose tissue (gWAT) after insulin administration ( n =6). Results are presented as mean±standard error of the mean. Statistical analyses were performed using one-sided two-way analysis of variance (ANOVA) (A, B) or one-sided one-way ANOVA (C). IPITT, intraperitoneal insulin tolerance test; CD, chow diet. a P <0.05, b P <0.01, c P <0.001 between indicated groups.

    Journal: Diabetes & Metabolism Journal

    Article Title: Phosphodiesterase 5 Inhibitor Improves Insulin Sensitivity by Regulating Adipose Tissue Macrophage Polarization in Diet-Induced Obese Mice

    doi: 10.4093/dmj.2024.0308

    Figure Lengend Snippet: Phosphodiesterase type 5 (PDE5) inhibitor (PDE5i) administration improves insulin sensitivity in high-fat diet (HFD)-induced obese mice. (A, B) Blood glucose levels measured by insulin tolerance tests performed after vehicle or PDE5i administration for 4 and 6 weeks ( n =6). (C) Western blot data and quantification of phospho AKT, total AKT, phosphor-4E-binding protein 1 (4EBP1), total 4EBP1, phospho S6, total S6, and β-actin in gonadal white adipose tissue (gWAT) after insulin administration ( n =6). Results are presented as mean±standard error of the mean. Statistical analyses were performed using one-sided two-way analysis of variance (ANOVA) (A, B) or one-sided one-way ANOVA (C). IPITT, intraperitoneal insulin tolerance test; CD, chow diet. a P <0.05, b P <0.01, c P <0.001 between indicated groups.

    Article Snippet: Subsequently, the membranes were incubated at 4°C with primary antibodies against phospho AKT (1:1,000, Cell Signaling Technology [CST], Danvers, MA, USA, #9271), total AKT (1:1,000, CST, #9272), phosphor-4E-binding protein 1 (4EBP1) (1:3,000, CST, #2855), total 4EBP1 (1:3,000, CST, #9644), phospho S6 (1:3,000, CST, #5364), total S6 (1:3,000, CST, #2217), and β-actin (1:1,000, Santa Cruz, Santa Cruz, CA, USA, #47778) for 16 hours.

    Techniques: Western Blot, Binding Assay